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Light Microscopy for
Biomedical Research
Workshop - September
22 to 26, 2008
University of North Carolina - Chapel Hill
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A one week workshop on
the use of light microscopy in biomedical research is being offered by the Michael Hooker Microscopy Facility,
UNC. The workshop is
intended to provide an introduction to the basic concepts of light microscopy
and its application to biological research with lectures, hands on sessions and industry
involvement.
Location: Bondurant and MacNider Halls, UNC, Chapel Hill, NC.
Tuition and Tuesday dinner: $700.
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| Monday - Sept 22 9:00 AM to 5:30 PM - Light Microscopy, modes of imaging, parts of a light
microscope, objectives, Kohler illumination, image formation, Hands on - parts of a microscope, care, digital
acquisition - alignment, Invited Talk - DIC/Phase Contrasts |
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| Tuesday - Sept 23 9:00 AM to 5:30 PM - Fluorescence, Jablonski diagram, contrast, filters, cross
talk, immuno labeling, cell labeling, hands on CCD cameras, noise, color/monochrome,
digital images, sampling, resolution, Nyquist , Dinner 6:30 PM |
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| Wednesday - Sept 24 9:00 AM to 5:30 PM - Confocal Microscopy, principles, scanning, PMTs,
AOTF, multichannel, 3D, projections, hands on confocal I - sensitivity, pinhole,
filters, averaging, zoom pixel size z-series, time lapse,
simultaneous/sequential, Colocalization, Invited Talk - Fluorescent Biosensors for Living Cells |
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| Thursday - Sept 25 9:00 AM to 5:30 PM - Advanced confocal, 2 photon, spinning disk, colocalization,
digital images, Fluorescence and FRAP, Hands on confocal II - multi-channel, FRAP,
rendering, 3D,
projections, 3-D deconvolution, Light sources |
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| Friday - Sept 26 9:00 AM to 4:00 PM - 3-D Image
deconvolution, 3-D Visualization, Tracking, 3-D
rendering, Visualization and Representation, Hands on - movies, scaling compression, resizing,
conversion, formatting for Power Point, imaging tools |
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Note: Topics are subject to change. Members of
Centers, Divisions & Laboratories which support MHMF via grants receive a discounted rate. |
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