Michael Hooker Microscopy Facility

microscopy.unc.edu

At the University of North Carolina

The Michael Hooker Microscopy Facility is a research microscopy facility in the Thurston Bowles building at the University of North Carolina, Chapel Hill.

The facility provides standard and advanced digital light microscopy and image processing resources to users from the UNC Chapel Hill campus on a fee for use basis.  Instrumentation and instruction are provided to enable users to acquire, process and analyze images from samples they have prepared, or we can acquire data with you.  Funding assistance may be obtained from the CTSA Pilot Grant program to Core Facilities (guidelines).

Information for new and current users - Users are required to complete the user information form and training with facility personnel for each microscope resource they wish use, or be assisted with scanning. Please contact Michael or Neal in order to schedule training or assisted scanning.  All users with wet or live samples must read this note.

• Mission Statement  |  Facility Policies  |  Charges
• New user/updated information form
• Information about MHmicroscopy logon accounts
• After hours access to Thurston Bowles 6129, 6123 & 6033a
• All users with wet or live samples must read this note

• Fluorescence stereo / macroscope with color & b/w cameras
• Volocity 3-D/4-D image visualization, analysis and deconvolution license
• Minsky -  Access is read only on \\minsky\users & \\minsky\users1.
• Zeiss 510 confocal transmitted light condenser has been replaced with a manual unit.
• Minsky - i.p. number is \\152.19.58.205 or use \\minsky.med.unc.edu  - Also note:  \\minsky\users & \\minsky\users1 access is read only

• Mar 08: Leica SP2 confocal - PMT voltage/gain is not producing overloaded pixels when turned up.  PMT circuit board(s) will be replaced in the week of March 15.  Be careful about saturated image regions region - turn PMT voltage down to unsaturate these regions.
• Mar 05: Zeiss 510 confocal - Intermittent focus delay.  Please find Michael or Neal immediately if you encounter the fine focus not responding while scanning or viewing by eye.  They would like to do diagnostic checks.
• Feb 24: Leica SP2 - Intermittent system crashes have abated.  See here for more details.

• Thurston Bowles building room 6129, 6033a, 6033 & 6123

• Users phone number: 966-7051 room 6129, 6033a, 6033, 6123

• Inverted Leica fluorescence microscope with digital cameras and DIC
• Upright Nikon fluorescence microscope and dissecting scope with color CCD camera
• Inverted Nikon Microscope, TE2000 multifunction time lapse with dual cameras
• Fluorescence Dissecting Stereo Microscope / Macroscope with b/w & color cameras

• Laser Scanning Confocal Microscope, Zeiss 510 meta
• Laser Scanning Confocal Microscope, Leica SP2 aobs
• Spinning Disk Confocal, Yokogawa
• Laser Scanning Confocal Microscope, LeicaTCS-4D

• Laser Micro-Dissection and capture, Leica AS-LMD
• Atomic Force Microscope
• Microscope stage heaters
• Printers: Color solid dye Phaser 8550  &  B/W laser double sided  HP1320
• High resolution color scanner: Epson Perfection 4180 Photo
• Humidified  Incubator
• Refrigerator (4 C) for sample and reagent storage

• Computer resources: File transfer - Network Connections - Accounts/Passwords
• Image Processing Workstations: Nomarski - Snell - Young - Zernike - Brewster
• Image Processing Software - Viewers - C-Imaging - Metamorph - Volocity - Image Surfer - Command prompt
• How To's - scale bars, basic image handling: colorize, overlay images, etc.

Copyright 2001-10   M. Chua, Office of Research, School of Medicine, University of North Carolina, Chapel Hill, NC 27599

Last updated  03/10/10